From the north: Autostrada A1 exit Valdarno-Montevarchi and after planning turn right, go into the direction of Siena. Between Montevarchi and Levane there is a big roundabout. Exit to direction Siena - Bucine. At that point, you don't need to enter any villages. You have to pass Bucine and Capannole (small village). After Capannole turn left to Badia Agnano, after 1 km you will find the signs to Villa Cini on your left, take the cypress lane and you are arrived.
From the south: Autostrada A1 exit Monte San Savino, then take the super strada and exit at Alberoro, follow the signs to Civitella in Val di Chiana, after Civitella go to Badia Agnano, after Badia Agnano go 1 km on your right, before Capannole, you will find the signs to Villa Cini.
The nearest train station is in Bucine but it is small and with a few stops, it is better to go to Arezzo or to Montevarchi, where you can take a taxi or rent a car.
The nearest airport is Amerigo Vespucci in Florence (about an hour from villa Cini). The airport of Galileo Galilei in Pisa is an hour and a half distance and the Airport Leonardo da Vinci in Rome is about two hours and a half from villa Cini. Once at the airport, you should rent a car.